Stenotrophomonas maltophilia was isolated from the rhizosphere of eggplant in the Nile Delta of Egypt, and its antagonistic potential against Ralstonia solanacearum race 3 biovar 2, the causal agent of potato brown rot, was in vitro evaluated on KB agar medium and in vivo on potato plants.
In vitro, four isolates of S. maltophilia (PD3531, PD3532, PD3533, and PD3534) appeared antagonistic. The isolate (PD3533) was screened as the most promising antagonist
for the in vivo tests. In the greenhouse, the antagonist was applied directly to soil or by bacterization of potato eyepieces.
Stenotrophomonas maltophilia significantly suppressed potato brown rot in Egyptian clay soil but not in Dutch clay soil. Survival of a rifampicin and
chloramphenicol-resistant S. maltophilia strain PD4560 was investigated in two pairs of clay soils, conventionally and organically managed, from Egypt and the Netherlands.
The survival of S. maltophilia was significantly less in Dutch than in Egyptian soils, while the converse occurred for R. solanacearum. These results are in agreement with those obtained in the in vivo biocontrol tests. In conclusion, S. maltophilia may be useful for control of brown rot in the area where it was originally isolated, the Nile Delta in Egypt. 相似文献
The genetic variation in seed weight, seed number per kg and seedling traits was compared among eight Acacia senegal provenances originating from the clay plain (east) and sand plains (west) of the gum belt in Sudan. The main objective of
this study was to identify germplasm sources of A. senegal that have a good seed germination capacity and seedling traits suitable for reforestation in the clay-soil part of the dryland
gum belt in the Blue Nile region in Sudan. A specific objective was to tentatively explore the adaptive strategy of A. senegal populations. Seventeen-week-old seedlings were planted in the field at spacing of 3 m × 3 m, giving 100 trees per plot and
replication; within a randomized complete block design with four replications. The experimental site was in the clay plain
region. Seed variables showed significant differences. Clay plain provenances showed considerable variation in seed weight
and seed number. They had the smallest seed weight but the highest seed number, while the sand (western) provenances had the
largest seed weight but lowest seed number. Seedling branch number, root length, root to shoot ratio and shoot dry weight
differed significantly among the provenances 12 weeks after germination. Clay provenances had the highest branch number and
shoot dry weight but the shortest roots and lowest root to shoot ratio. This was interpreted as showing better adaptation
to the site in these local provenances in comparison to those originating from the western sandy soil regions. High positive
correlations were observed between seedling variables, such as root nodule and branch numbers; this could be used for early
selection. The variation was greater between provenance groups than within them, suggesting that especially selection among
groups would yield genetic gain. 相似文献
Within-field variations in plant-available soil nitrogen (N) are likely to be affected by differences in soil characteristics. To study this, a 3- year field investigation was conducted during 1998-2000 on a 15 ha arable field in Sweden with considerable within-field soil texture variability. In 34 plots soil N uptake by crops, net nitrogen mineralization (Nm) during the growing season and soil mineral N in spring and shortly after harvest were determined. Beside these parameters, topography, soil organic matter content (SOM), clay content, pH(H 2 O) and grain yield were recorded. The variations in Nm were considerably large both within the field and between years. The within-field variation in Nm could partly be explained by the variation in SOM and clay content (adjusted coefficient of determination = 0.23, P <0.001). The pattern in Nm differed between years, partly because of seasonal variations in soil moisture. For these reasons, the pattern of Nm is difficult to predict without seasonal adjustments. 相似文献
The effects of wheel traffic on soil surface hydraulic properties, and consequent effects on erosion, following planting of vegetable crops in beds have not been widely studied. This paper describes two trials to quantify how wheel tracks influence infiltration and erosion rates, and assesses the value of cultivating wheel tracks for reducing erosion. The trials were carried out under natural rainfall, on Dystric Nitosols with clay loam texture and strong, stable structure. Net rates of erosion from onion (Allium cepa L.) beds with cultivated or uncultivated inter-bed wheel tracks were measured with erosion pins and repeat topographic surveys of sediment trapped in silt fences. Infiltration rates in onion beds, cultivated and uncultivated wheel tracks, and changes in infiltration rates through winter, spring, and summer, were measured using the double-ring, ponded-water method.
Differences in erosion rate were only measured in the second trial in which erosion rate from the uncultivated treatment was 21 Mg ha−1, compared to 1 Mg ha−1 for the cultivated treatment. Erosion occurred through mobilisation of soil along the edge and base of the wheel tracks, with no evidence of erosion of the onion beds. Most of the eroded soil comprised soil aggregates, with 75% between 0.25 and 4 mm in diameter, suggesting soil was transported in runoff along the wheel tracks as stable aggregates. Uncultivated wheel tracks had very low infiltration rates compared to onion beds and cultivated wheel tracks. The differences in infiltration rates between cultivated and uncultivated wheel tracks were consistent in both trials, with minor differences due to rainfall patterns and the implements used to cultivate wheel tracks. There were clear trends in infiltration rates through time, with rates in the uncultivated wheel tracks increasing during the growing season from 1.4×10−7 to 2.1×10−5 ms−1 and in onion beds from 1.1×10−4 to 2.5×10−4 ms−1, while rates in the cultivated wheel tracks decreased from 1.7×10−2 to 2.4×10−3 ms−1. The major increase of infiltration rate in uncultivated wheel tracks occurred after October when the soil surface began to dry out, and frequent wetting and drying cycles caused the compacted surface soil to crack. Most erosion occurred in the winter/early spring period when storm frequency and rainfall intensity was highest, and infiltration rates in the uncultivated wheel tracks lowest. Cultivating wheel tracks is a simple and effective practice to increase infiltration of rainfall and reduce erosion rates on clay-rich, strongly structured soils. 相似文献